Load all required libraries.
library(tidyverse)
## -- Attaching packages --------------------------------------- tidyverse 1.3.1 --
## v ggplot2 3.3.5 v purrr 0.3.4
## v tibble 3.1.3 v dplyr 1.0.7
## v tidyr 1.1.3 v stringr 1.4.0
## v readr 2.0.0 v forcats 0.5.1
## -- Conflicts ------------------------------------------ tidyverse_conflicts() --
## x dplyr::filter() masks stats::filter()
## x dplyr::lag() masks stats::lag()
library(plotly)
##
## Attaching package: 'plotly'
## The following object is masked from 'package:ggplot2':
##
## last_plot
## The following object is masked from 'package:stats':
##
## filter
## The following object is masked from 'package:graphics':
##
## layout
library(broom)
Read in raw data from RDS.
raw_data <- readRDS("./year2.RDS")
Make a few small modifications to names and data for visualizations.
final_data <- raw_data %>% mutate(log_copy_per_L = log10(mean_copy_num_L)) %>%
rename(Facility = wrf) %>%
mutate(Facility = recode(Facility,
"NO" = "WRF A",
"MI" = "WRF B",
"CC" = "WRF C"))
Seperate the data by gene target to ease layering in the final plot
#make three data layers
only_positives <<- subset(final_data, (!is.na(final_data$Facility)))
only_n1 <- subset(only_positives, target == "N1")
only_n2 <- subset(only_positives, target == "N2")
only_background <<-final_data %>%
select(c(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke)) %>%
group_by(date) %>% summarise_if(is.numeric, mean)
#specify fun colors
background_color <- "#7570B3"
seven_day_ave_color <- "#E6AB02"
marker_colors <- c("N1" = '#1B9E77',"N2" ='#D95F02')
#remove facilty C for now
#only_n1 <- only_n1[!(only_n1$Facility == "WRF C"),]
#only_n2 <- only_n2[!(only_n2$Facility == "WRF C"),]
only_n1 <- only_n1[!(only_n1$Facility == "WRF A" & only_n1$date == "2020-11-02"), ]
only_n2 <- only_n2[!(only_n2$Facility == "WRF A" & only_n2$date == "2020-11-02"), ]
Build the main plot
#first layer is the background epidemic curve
p1 <- only_background %>%
plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~new_cases_clarke,
type = "bar",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Daily Cases: ', new_cases_clarke),
alpha = 0.5,
name = "Daily Reported Cases",
color = background_color,
colors = background_color,
showlegend = FALSE) %>%
layout(yaxis = list(title = "Clarke County Daily Cases", showline=TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#renders the main plot layer two as seven day moving average
p1 <- p1 %>% plotly::add_trace(x = ~date, y = ~X7_day_ave_clarke,
type = "scatter",
mode = "lines",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Seven-Day Moving Average: ', X7_day_ave_clarke),
name = "Seven Day Moving Average Athens",
line = list(color = seven_day_ave_color),
showlegend = FALSE)
#renders the main plot layer three as positive target hits
p2 <- plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n1,
symbol = ~Facility,
marker = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n2,
symbol = ~Facility,
marker = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(yaxis = list(title = "SARS CoV-2 Copies/L",
showline = TRUE,
type = "log",
dtick = 1,
automargin = TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#adds the limit of detection dashed line
p2 <- p2 %>% plotly::add_segments(x = as.Date("2021-06-30"),
xend = ~max(date + 10),
y = 3571.429, yend = 3571.429,
opacity = 0.35,
line = list(color = "black", dash = "dash")) %>%
layout(annotations = list(x = as.Date("2021-06-30"), y = 3.8, xref = "x", yref = "y",
text = "Limit of Detection", showarrow = FALSE))
p1
p2
Combine the two main plot pieces as a subplot
#seperate n1 and n2 frames by site
#n1
wrf_a_only_n1 <- subset(only_n1, Facility == "WRF A")
wrf_b_only_n1 <- subset(only_n1, Facility == "WRF B")
wrf_c_only_n1 <- subset(only_n1, Facility == "WRF C")
#n2
wrf_a_only_n2 <- subset(only_n2, Facility == "WRF A")
wrf_b_only_n2 <- subset(only_n2, Facility == "WRF B")
wrf_c_only_n2 <- subset(only_n2, Facility == "WRF C")
#rejoin the old data frames then seperate in to averages for each plant.
wrfa_both <- full_join(wrf_a_only_n1, wrf_a_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "day", "log_copy_per_L")
wrfb_both <- full_join(wrf_b_only_n1, wrf_b_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "day", "log_copy_per_L")
wrfc_both <- full_join(wrf_c_only_n1, wrf_c_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "day", "log_copy_per_L")
#get max date
maxdate <- max(wrfa_both$date)
mindate <- min(wrfa_both$date)
Build loess smoothing figures figures
This makes the individual plots
#**************************************WRF A PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_botha <- ggplot(wrfa_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_botha<<-..y..), method = "loess", color = '#1B9E77',
span = 0.6, n = 64)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_botha
## `geom_smooth()` using formula 'y ~ x'
fit_botha
## [1] 11.56357 11.59167 11.62069 11.65057 11.68125 11.71267 11.74477 11.77777
## [9] 11.81180 11.84666 11.88216 11.91810 11.95428 11.99237 12.03333 12.07587
## [17] 12.11870 12.16052 12.20005 12.23599 12.26774 12.29635 12.32306 12.34908
## [25] 12.37564 12.40397 12.43529 12.46976 12.50597 12.54278 12.57903 12.61358
## [33] 12.64530 12.67303 12.69480 12.71072 12.72281 12.73309 12.74358 12.75627
## [41] 12.77320 12.77566 12.78010 12.80937 12.84779 12.89225 12.93962 12.98680
## [49] 13.03067 13.06810 13.10089 13.13227 13.16134 13.18719 13.20892 13.22563
## [57] 13.23639 13.24200 13.24373 13.24138 13.23477 13.22372 13.20803 13.18752
#assign fits to a vector
both_trenda <- fit_botha
#extract y min and max for each
limits_botha <- ggplot_build(extract_botha)$data
## `geom_smooth()` using formula 'y ~ x'
limits_botha <- as.data.frame(limits_botha)
both_ymina <- limits_botha$ymin
both_ymaxa <- limits_botha$ymax
#reassign dataframes (just to be safe)
work_botha <- wrfa_both
#fill in missing dates to smooth fits
work_botha <- work_botha %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_botha <- work_botha$date
#create a new smooth dataframe to layer
smooth_frame_botha <- data.frame(date_vec_botha, both_trenda, both_ymina, both_ymaxa)
#WRF A
#plot smooth frames
p_wrf_a <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_botha, y = ~both_trenda,
data = smooth_frame_botha,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha,
'</br> Median Log Copies: ', round(both_trenda, digits = 2)),
line = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_botha, ymin = ~both_ymina, ymax = ~both_ymaxa,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxa, digits = 2),
'</br> Min Log Copies: ', round(both_ymina, digits = 2)),
name = "",
fillcolor = '#1B9E77',
line = list(color = '#1B9E77')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF A") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfa_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#1B9E77', size = 6, opacity = 0.65))
p_wrf_a
save(p_wrf_a, file = "./site_objects/wrf_a_year2.rda")
#**************************************WRF B PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothb <- ggplot(wrfb_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothb<<-..y..), method = "loess", color = '#D95F02',
span = 0.6, n = 64)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothb
## `geom_smooth()` using formula 'y ~ x'
fit_bothb
## [1] 10.70669 10.80291 10.89622 10.98667 11.07433 11.15925 11.24149 11.32034
## [9] 11.39557 11.46787 11.53797 11.60658 11.67441 11.73845 11.79649 11.85046
## [17] 11.90229 11.95392 12.00727 12.06427 12.12947 12.20372 12.28345 12.36505
## [25] 12.44493 12.51950 12.58518 12.64290 12.69567 12.74300 12.78438 12.81931
## [33] 12.84727 12.86776 12.87777 12.87695 12.86878 12.85677 12.84441 12.83519
## [41] 12.83260 12.81865 12.80628 12.81783 12.83997 12.86915 12.90179 12.93430
## [49] 12.96313 12.98469 12.99989 13.01216 13.02169 13.02867 13.03329 13.03573
## [57] 13.03619 13.03463 13.03081 13.02465 13.01606 13.00495 12.99123 12.97481
#assign fits to a vector
both_trendb <- fit_bothb
#extract y min and max for each
limits_bothb <- ggplot_build(extract_bothb)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothb <- as.data.frame(limits_bothb)
both_yminb <- limits_bothb$ymin
both_ymaxb <- limits_bothb$ymax
#reassign dataframes (just to be safe)
work_bothb <- wrfb_both
#fill in missing dates to smooth fits
work_bothb <- work_bothb %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothb <- work_bothb$date
#create a new smooth dataframe to layer
smooth_frame_bothb <- data.frame(date_vec_bothb, both_trendb, both_yminb, both_ymaxb)
#WRF B
#plot smooth frames
p_wrf_b <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothb, y = ~both_trendb,
data = smooth_frame_bothb,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb,
'</br> Median Log Copies: ', round(both_trendb, digits = 2)),
line = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothb, ymin = ~both_yminb, ymax = ~both_ymaxb,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxb, digits = 2),
'</br> Min Log Copies: ', round(both_yminb, digits = 2)),
name = "",
fillcolor = '#D95F02',
line = list(color = '#D95F02')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF B") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfb_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#D95F02', size = 6, opacity = 0.65))
p_wrf_b
save(p_wrf_b, file = "./site_objects/wrf_b_year2.rda")
#**************************************WRF C PLOT********************************************** #add trendlines #extract data from geom_smooth # *********************************span 0.6*********************************** #*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothc <- ggplot(wrfc_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothc<<-..y..), method = "loess", color = '#E7298A',
span = 0.6, n = 64)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothc
## `geom_smooth()` using formula 'y ~ x'
fit_bothc
## [1] 10.59325 10.71609 10.83274 10.94339 11.04822 11.14741 11.24114 11.32988
## [9] 11.41367 11.49208 11.56469 11.63106 11.69076 11.73952 11.77565 11.80255
## [17] 11.82363 11.84232 11.86203 11.88616 11.91379 11.94107 11.96706 11.99080
## [25] 12.01134 12.02775 12.03908 12.04541 12.04946 12.05416 12.06244 12.07725
## [33] 12.10153 12.13821 12.19072 12.25596 12.32711 12.39737 12.45992 12.50794
## [41] 12.53462 12.51661 12.49589 12.49694 12.48657 12.46952 12.45054 12.43438
## [49] 12.42579 12.42952 12.44581 12.46987 12.49894 12.53022 12.56093 12.58829
## [57] 12.60952 12.62710 12.64505 12.66292 12.68027 12.69665 12.71162 12.72473
#assign fits to a vector
both_trendc <- fit_bothc
#extract y min and max for each
limits_bothc <- ggplot_build(extract_bothc)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothc <- as.data.frame(limits_bothc)
both_yminc <- limits_bothc$ymin
both_ymaxc <- limits_bothc$ymax
#reassign dataframes (just to be safe)
work_bothc <- wrfc_both
#fill in missing dates to smooth fits
work_bothc <- work_bothc %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothc <- work_bothc$date
#create a new smooth dataframe to layer
smooth_frame_bothc <- data.frame(date_vec_bothc, both_trendc, both_yminc, both_ymaxc)
#WRF C
#plot smooth frames
p_wrf_c <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothc, y = ~both_trendc,
data = smooth_frame_bothc,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc,
'</br> Median Log Copies: ', round(both_trendc, digits = 2)),
line = list(color = '#E7298A', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothc, ymin = ~both_yminc, ymax = ~both_ymaxc,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxc, digits = 2),
'</br> Min Log Copies: ', round(both_yminc, digits = 2)),
name = "",
fillcolor = '#E7298A',
line = list(color = '#E7298A')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF C") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfc_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#E7298A', size = 6, opacity = 0.65))
p_wrf_c
save(p_wrf_c, file = "./site_objects/wrf_c_year2.rda")
keeping in case
#save(wrfa_both, file = "./plotly_objs/wrfa_both.rda")
#save(wrfb_both, file = "./plotly_objs/wrfb_both.rda")
#save(wrfc_both, file = "./plotly_objs/wrfc_both.rda")
#save(date_vec_botha, file = "./plotly_objs/date_vec_botha.rda")
#save(date_vec_bothb, file = "./plotly_objs/date_vec_bothb.rda")
#save(date_vec_bothc, file = "./plotly_objs/date_vec_bothc.rda")
#save(both_ymina, file = "./plotly_objs/both_ymina.rda")
#save(both_ymaxa, file = "./plotly_objs/both_ymaxa.rda")
#save(both_yminb, file = "./plotly_objs/both_yminb.rda")
#save(both_ymaxb, file = "./plotly_objs/both_ymaxb.rda")
#save(both_yminc, file = "./plotly_objs/both_yminc.rda")
#save(both_ymaxc, file = "./plotly_objs/both_ymaxc.rda")